NOD2 stimulation enhances the innate immunity against Mycobacterium tuberculosis in human alveolar macrophages
10.3969/j.issn.1673-4130.2015.10.008
- VernacularTitle:NOD2信号对人肺泡巨噬细胞抗结核分枝杆菌活性的影响及机制研究
- Author:
Daqing YANG
;
Liping SHI
;
Pushan ZHANG
- Publication Type:Journal Article
- Keywords:
nucleotide-binding oligomerization domain 2;
Mycobacterium tuberculosis;
macrophage
- From:
International Journal of Laboratory Medicine
2015;(10):1338-1340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of nucleotide‐binding oligomerization domain 2(NOD2) stimulation in innate immuni‐ty against M ycobacterium tuberculosis .Methods Plate counting as used to evaluate the effect of resisting M ycobacterium tubercu‐losis in human alveolar macrophages .Intracellular NOD2 expression were detected by flow cytometry .Quantitative real‐time PCR was performed to determine the NOD2 ,inducible nitric oxide synthase(iNOS) ,and DEF4B mRNA expression levels using the com‐parative threshold cycle method of relative quantitation .Reactive oxygen species(ROS) were detected by the DFCH probe .Results NOD2 stimulation enhanced the control of intracellular mycobacterial growth in human alveolar macrophages .Although ROS con‐centration did not changed ,the secretion of Nitro Oxygen and the expression of cathelicidin DEFB4 were significantly increased fol‐lowing NOD2 stimulation in human alveolar macrophages .Conclusion NOD2 stimulation may be involved in the early innate con‐trol of Mycobacterium tuberculosis primary infections inducing the generation of Nitro Oxygen and the peptides cathelicidin DEFB4 .
