Study of NO and FOXP3 in patients with pulmonary tuberculosis
10.3969/j.issn.1673-4130.2015.10.019
- VernacularTitle:肺结核患者 NO 和FOXP3表达水平变化及意义
- Author:
Liyan SHEN
;
Weihong SHEN
;
Lan ZHU
- Publication Type:Journal Article
- Keywords:
nitric oxide;
forkhead box protein 3;
pulmonary tuberculosis
- From:
International Journal of Laboratory Medicine
2015;(10):1364-1365
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Study the expressions and significance of nitric oxide (NO) and forkhead box protein 3 (FOXP3) in patients with pulmonary tuberculosis .Methods Serum NO levels were measured by Griess colorimetric reaction .Flow cytometry was used to determine the number of CD4+CD25+ T cells in peripheral blood .The expression of FOXP3 mRNA was measured by real‐time polymerase chain reaction .Results Serum NO level in patients was (15 .71 ± 1 .26)μmol/L ,higher than the (5 .45 ± 0 .98)μmol/L of healthy controls (P<0 .05) .CD4+ CD25+ T cells comprised (4 .57 ± 0 .85)% of CD4+ T cells in patients ,higher than the (1 .83 ± 0 .49)% in healthy controls (P<0 .05) .CD4+ CD25+ T cells in the peripheral blood of patients with pulmonary tuberculosis highly expressed FOXP3 .Conclusion Patients with pulmonary tuberculosis could be with an increased level of NO and FOXP3 ,which might have important role in the pathogenesis of pulmonary tuberculosis .
