Aquaporin1 expression in glioma patients and its potential func-tion in glioma progression
10.3969/j.issn.1000-8179.20150336
- VernacularTitle:Aquaporin1在胶质瘤中的表达及其临床意义
- Author:
Jia HE
;
Wenliang LI
;
Feng GU
;
Yongjie MA
- Publication Type:Journal Article
- Keywords:
glioma;
aquaporin1;
proliferation;
invasion;
survival
- From:
Chinese Journal of Clinical Oncology
2015;(10):493-498
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of aquaporin1 (AQP1) in human glioma tissues and its relationship with the clini-copathological parameters and prognosis of this tumor. This study also observed the function of AQP1 in the proliferation and invasion of LN229 glioblastoma cells. Methods:The expression of AQP1 in 135 cases of glioma was detected by immunohistochemical meth-od, and the correlation between AQP1 and pathological features of glioma was analyzed. The relationship of AQP1 with survival was al-so investigated using 103 specimens with complete clinical data. AQP1 was successfully transfected into LN229 cells with lentiviral vector, and the expression of AQP1 protein was tested by Western blot. Cell proliferation was detected by using methyl thiazolyl tetrazo-lium assay, whereas cell invasion was determined by Transwell assay. Results:The expression of AQP1 was positively correlated with pathological grading. High AQP1 expression was associated with poor prognosis (P<0.05). Moreover, the overexpression of AQP1 can significantly increase the proliferation and invasion of LN229 cells (P<0.05). Conclusion:AQP1 is closely associated with the progres-sion of glioma. Upregulation of the AQP1 expression promoted the proliferation and metastasis of glioma cells. These findings indicat-ed that AQP1 can function as a therapeutic target for glioma in future research.
