Effects and related mechanisms of preconditioning with puerarin on hippocampus CA1 region neuronal injury after focal cerebral ischemia-reperfusion
- VernacularTitle:葛根素预处理对局灶性脑缺血再灌注大鼠海马 CA1区神经元损伤的影响及其机制
- Author:
Xueli YIN
;
Li GUI
;
Zhen LI
- Publication Type:Journal Article
- Keywords:
puerarin;
ischemia-reperfusion injury;
neuroprotection;
Caspase-3;
Caspase-9
- From:
Acta Universitatis Medicinalis Anhui
2015;(6):723-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of puerarin preconditioning on hippocampal CA1 region neuronal injury and further explore its mechanisms. Methods 30 male Sprague-Dawley rats were randomly assigned into sham, model,and puerarin preconditioning ischemia-reperfusion groups. The middle cerebral artery was occluded for 60 min by an intraluminal filament before reperfusion to induce transient focal cerebral ischemia reperfusion rats. Puer-arin preconditioning groups rats received puerarin(100 mg / kg,i. p)1 h before ischemia. The histological changes of hippocampal CA1 region neurons were measured by HE staining on the 5th day after reperfusion. The expressions of Caspase-3 and Caspase-9 proteins in the CA1 region were examined by immunohistochemistry staining. Results The results of HE staining showed that,compared with the model group,puerarin preconditioning significantly re-duced the number of cerebral ischemia induced injury of hippocampal CA1 region neurons(P < 0. 05). Immunohis-tochemistry data showed that the expressions of Caspase-3 and Caspase-9 in the hippocampal CA1 region of puerarin preconditioning group were significantly lower than that of model group(P < 0. 05). Conclusion The results indi-cate that puerarin preconditioning 1 h before ischemia exerts neuroprotective effects on focal cerebral ischemia-reperfusion induced neuronal injury. The neuroprotective mechanisms of puerarin may attribute to inhibiting the ex-pressions of Caspase-3 and Caspase-9 protein.
