Applications of microsatellite marker technology in the genetic structure research for closed colony of Brandt’ s voles
10.3969/j.issn.1671.7856.2015.007.008
- VernacularTitle:微卫星标记在布氏田鼠封闭群遗传结构研究中的应用
- Author:
Man ZHANG
;
Haixia SHI
;
Mingjing SONG
- Publication Type:Journal Article
- Keywords:
Brandt’ s vole;
Closed colony;
Genetic structure;
Microsatellite DNA marker
- From:
Chinese Journal of Comparative Medicine
2015;(7):34-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective Analysis of the genetic structure stability of Brandt ’ s vole ( lasiopodomys brandtii ) in closed colony using microsatellite marker technology.Methods Genomic DNA was extracted from tail tip using high-concentration-salt precipitation methods.Marked with fluorescent tags( Fam) , 7 microsatellite primers were filtered out by PCR, and the DNA structure of three consecutive generations of Brandt’ s vole was analyzed by microsatellite marker. Results By the analysis of the average heterozygosity and polymorphism information content, Brandt’ s vole populations maintaineda closed group of qualified genetic structure.Conclusions The present results show that the closed group of Brandt’ s vole species in our laboratory maintain a stable genetic structure.
