Study on the expression of ETFβin diabetic nephropathy and its relationship with lipotoxicity
10.3969/j.issn.1671.7856.2015.005.002
- VernacularTitle:糖尿病肾病 ETFβ表达变化与脂毒性关系研究
- Author:
Hua WANG
;
Haojun ZHANG
;
Tingting ZHAO
;
Meihua YAN
;
Xi DONG
;
Sifan SUN
;
Bingxuan ZHANG
;
Ping LI
- Publication Type:Journal Article
- Keywords:
ETFβ;
Diabetic nephropathy;
Fatty acid;
Apoptosis
- From:
Chinese Journal of Comparative Medicine
2015;(5):5-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression change of ETFβin diabetic nephropathy rats and study the role of ETFβin fatty acid-induced apoptosis in renal tubules.Methods Diabetic nephropathy model was established by intraperitoneal injection of streptozotocin and unilateral nephrectomy.In vivo ETFβexpression was detected in renal cortex, as well as tubular injury evaluated.In vitro fatty acid-induced apoptosis in renal tubular cells NRK 52E model was established and ETFβrecombinant plasmid was constructed to be transfected into NRK 52E cells and furtherly to observe the effect of ETFβover-expression on the fatty acid-induced apoptosis.Results In the rats model of diabetic nephropathy induced by streptozotocin injection and unilateral nephrectomy, ETFβmRNA and protein expression were decreased as obvious tubular damage occurred.Fatty acids could induce apoptosis in NRK 52E, and ETFβover-expression reduced the apoptosis.Conclusion The expression of ETFβis decreased in diabetic nephropathy model , and ETFβover-expression can reduce apoptosis induced by fatty acid in renal tubular cells.
