Comparative study of severe acute pancreatitis model in rats with three different severity
10.3969/j.issn.1671.7856.2015.005.005
- VernacularTitle:三种不同严重程度大鼠重症胰腺炎模型比较研究
- Author:
Lin YUAN
;
Bingduo ZHOU
;
Xiaosu WANG
;
Zhonghua WU
;
Jing KONG
;
Tingting XU
;
Hongwei WANG
;
Shengliang ZHU
;
Shengquan FANG
;
Li ZHOU
;
Chunfang LIU
;
Xiulian ZHANG
;
Yi WANG
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
The method of retrograde injection into the biliopancreatic duct;
Sodium taurocholate;
Model,animal
- From:
Chinese Journal of Comparative Medicine
2015;(5):20-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective to provide the evidence for inducing the SAP model in rats with proper concentration of sodium taurocholate.Methods 60 SD rats were divided into sham operated group, group of 1.5% in concentration, group of 3.5% in concentration and group of 5% in concentration randomly, while the SAP model was induced by the sodium taurocholate concentration of 1.5%,3.5% and 5% with the method of retrograde injection into the biliopancreatic duct.To calculate the mortality of different groups, measure the serum amylase, tumor necrosis factor -α(TNF -α) and interleukin -6 (IL -6),and to observe the pancreatic pathological scores of HE staining in rats.Results The mortality in group of 5% in concentration has a significant ascending compared with group of 1.5% in concentration, while the serum amylase, tumor necrosis factor -α(TNF -α) , interleukin -6( IL -6), pathological score of hemorrhage and acinar necrosis in group of 5% in concentration have a significant ascending compared with group of 1.5% in concentration and group of 3.5% in concentration.Conclusions A better SAP model may be induced by sodium taurocholate with the concentration of 5% by the method of retrograde injection into the biliopancreatic duct, which may accord with the physiological and pathological manifestation of SAP.
