Production and phenotype identification of specific expressed green fluorescent protein in male mice germ cells
10.3969/j.issn.1671.7856.2015.005.007
- VernacularTitle:雄性生殖细胞特异性表达绿色荧光蛋白小鼠的繁殖及其表型鉴定
- Author:
Zhiru WANG
;
Jun LI
;
Xiaomei LIU
;
Honglian WU
;
Desheng ZHU
- Publication Type:Journal Article
- Keywords:
Testis;
Specificity;
Gene recombination;
Green fluorescent protein
- From:
Chinese Journal of Comparative Medicine
2015;(5):29-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study is production of organ specific animal model for studying reproductive toxicity in mice.Methods F1 generation was gotten by mating the Ddx4 -cre transgenic male mice with the Rosa26mT/mG transgenic female mice.F1 offspring and its parents phenotype was screened by molecular biological, histopathological and in vivo imaging technology.Results At molecular level, specific DNA fragment was only found in testis of F1 offspring; At the organ level, the expression of green fluorescent protein could only be observed in testis of F1 offspring; Testicular frozen sections and sperm fluorescence observation showed that green fluorescent protein were mainly expressed in the germ cell lineage such as secondary spermatocyte and spermatocyte and spermatozoon.Conclusions The production of the mice with specific germ cell expressed green fluorescent protein by Cre/loxP recombination system were built successfully.
