Polysaccharide from eggs of S. nudus (SEP) prevents cardiomyocyte hypertrophy induced by high glucose and high insulin in vitro in neonatal rat
10.3969/j.issn.1001-1978.2015.06.021
- VernacularTitle:海胆黄多糖SEP抑制高糖高胰岛素诱导的大鼠乳鼠心肌细胞肥大
- Author:
Qingfeng ZHOU
;
Chunhui LIU
;
Hongxin WANG
- Publication Type:Journal Article
- Keywords:
myocardial cell;
hypertrophy;
neonatal rat;
high glucose and high insulin;
polysaccharide;
SEP
- From:
Chinese Pharmacological Bulletin
2015;(6):844-847
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the protective effects of SEP on the hypertrophic myocardial cells induced with high glucose and high insulin and the mechanism. Methods The protein content was assayed with Lowrys meth-od;the cardiomyocytes area was measured by computer photograph analysis system;the expression of ANF and PPAR-α was determined by RT-PCR;APS was select-ed as control drug. Results Compared with conctrol group,the protein content,cardiomyocytes area and the expression of ANF and PPAR-α of high glucose and high insulin group were significantly increased. Com-pared with conctrol group,the SEP of different dosages were able to decrease the protein content, area, the expression of ANF mRNA and PPAR-α mRNA of cul-tured hypertrophic myocadial cells induced with high glucose and high insulin. Conclusion SEP can pre-vent cardiomyocyte hypertrophy induced by high glu-cose and high insulin, which is related to its inhibition on PPAR-α signaling path.
