Inhibitory effect of gossypol on migration of gastric carcinoma cell lines through Akt/β-catenin passway
10.3969/j.issn.1001-1978.2015.06.024
- VernacularTitle:棉酚通过Akt/β-catenin通路抑制胃癌细胞迁移
- Author:
Dan YANG
;
Li WANG
;
Zhu LI
;
Zhaoyang LIU
;
Chang LIU
;
Li GUO
;
Jia FU
;
Rong QI
;
Junping WANG
- Publication Type:Journal Article
- Keywords:
gossypol;
gastric carcinoma;
Akt;
β-cate-nin;
cyclin D1;
MMP-2;
E-cadherin;
vimentin
- From:
Chinese Pharmacological Bulletin
2015;(6):860-864
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the inhibitory effects of gossypol on migration in gastric carcinoma cell lines and its mechanisms. Methods Gastric carcinoma cells were treated with gossypol at different concentra-tions. The effects of gossypol on cells proliferation were measured using the MTT assay. The migration of gas-tric carcinoma cells was detected by transwell assay. The activation of Akt/β-catenin pathway and the ex-pressions of pathway related proteins ( p-Akt,β-cate-nin, cyclin D1, MMP-2, E-cadherin and vimentin ) were detected by Western blot. Results Gossypol treatment could significantly inhibit the proliferation of gastric carcinoma cells in a dose-dependent manner. Transwell assay showed that the migration ability of
gastric carcinoma cells was significantly decreased. The inhibitory effect of gossypol on cells migration was more significant than the effect of gossypol on cells prolifera-tion. Compared with the control group, treatment with gossypol significantly suppressed the expressions of p-Akt,β-catenin, cyclin D1, MMP-2 and vimentin pro-tein, whereas the expression of E-cadherin was signifi-cantly up-regulated in gastric carcinoma cells in a dose-dependent manner. Conclusion These results demonstrate that gossypol represses cell migration of gastric carcinoma cells through the down-regulation of the activity of Akt/β-catenin pathway.
