Expression of TPX2 in breast cancer tissue and their clinical significances
10.13315/j.cnki.cjcep.2015.05.011
- VernacularTitle:乳腺癌组织中TPX2蛋白的表达及其临床意义
- Author:
Chunmei ZHANG
;
Min LI
;
Shixian ZHOU
- Publication Type:Journal Article
- Keywords:
breast neoplasm;
TPX2 protein;
TPX2 mRNA;
immunohistochemistry
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(5):528-531
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression of TPX2 in breast cancer, analyze the correlation between its expression and clinico-pathologic characteristics and explore the significances of TPX2 in the treatment and prognosis of breast cancer. Methods Expression of TPX2 in breast cancer tissues, adjacent tissue of cancer and normal breast tissue was examined by immunohistochenistry SP method. The mRNA expression of TPX2 in breast cancer tissues, adjacent tissue of cancer and normal breast tissue was detected by reverse-transcription polymerase chain reaction ( RT-PCR) . Results The expression of TPX2 is significantly higher in breast cancer tissues (75. 86%) compared with adjacent tissue of cancer (40. 00%) and normal breast tissue (5. 00%). And the expression of TPX2 cor-related with tumor grade and lymph node metastasis (P<0. 01), while unrelated to age and clinical stage (P>0. 05). The expression of TPX2 mRNA is also significantly higher in breast cancer tissues ( 1. 465 7 ± 0. 136 6 ) compared with adjacent tissue of cancer (0. 923 3 ±0. 114 8) and normal breast tissue (0. 326 9 ±0. 097 3) (P<0. 01). Conclusion High expression of TPX2 in breast cancer correlated with tumor grade and lymph node metastasis, so TPX2 might be a risk factor in the tumorigenesis, progression and lymph node metastasis in breast cancer.
