Assessing the quality of rodent laboratory animals in Beijing area by pathological diagnosis in October 2014
10.3969/j.issn.1671.7856.2015.005.016
- VernacularTitle:2014年北京地区啮齿类实验动物质量的病理学评价
- Author:
Chao LI
;
Haodi DONG
;
Linkai XU
;
Chunyu WANG
;
Zhiqi SONG
;
Chunfa LIU
;
Chaosi LI
;
Ruichao YUE
;
Guangyu CHENG
;
Huajia ZHAO
;
Deming ZAO
;
Xiaomin YIN
;
Xiangmei ZHOU
;
Lifeng YANG
- Publication Type:Journal Article
- Keywords:
Laboratory rodents;
Pathological diagnosis;
Organ diseases;
Oil red-ostain;
PAS stain
- From:
Chinese Journal of Comparative Medicine
2015;(5):67-70
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the health status of rodent laboratory animals by pathological diagnosis, our lab has being take apart in investigating the quality of laboratory animals in Beijing area for years and offer some advices for standardized breeding to ensure accurate results of scientific research.This paper focuses on the analysis of laboratory rodent samples that collected in October 2014.Methods We collected the heart, liver, spleen, lung, kidney, large intestine and small intestine, and put these organs into 10%Calcium formaldehyde solution for fixation, and then prepared into two different sections for optical microscopy observation including all paraffin specimens stained with H&E and the frozen sections stained with Oil Red-O and PAS.Results The vast majority of laboratory rodents were up to standard, but there still a problem in individual units.The main problem is liver and lung disease.The rate of Hepatocyte swellingis 6%(mouse), 2.5% (rat), 8.2% (guinea pig), moreover part of them were lipidosis, according to Oil Red-O stain.the mainly problem of lung is congestion ,edema and Interstitial pneumonia ,the detectable rate of pulmonarydiseases is 15.5%(guinea pig).Conclusions The vast majority of laboratory rodents were pathologically diagnosed as healthy animals.The liver disease may be caused by improper feeding.And disease of lung may led by haze, unqualified bedding and low temperature.
