The Evaluation of Anti-tumor Effect of CTL Induced by Oxidized Mannan-modified Tumor Cells Vaccine
- VernacularTitle:氧化型甘露聚糖修饰的肿瘤细胞疫苗诱导CTL的抗肿瘤免疫效果评价
- Author:
Jia SU
;
Yutong WANG
;
Xinhui QI
;
Yan LI
;
Di WU
;
Yunpeng LIU
;
Youhong JIANG
- Publication Type:Journal Article
- Keywords:
renal clear cell carcinoma;
dendritic cells;
mannose receptor
- From:
Journal of China Medical University
2015;(5):420-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study to take the oxidized Mannan?modified 786?0 in renal clear cell carcinom as tumor cells antigen to sensitize Dendrit?ic cells(DC)and to observe the its killing effect on renal clear cell carcinoma of CTLs induced. Methods Getting the peripheral blood mononucle?ar cells from the volunteers,and then to be stimulated to turn to be maturation by GM?CSF and IL?4 in vitro. Taking the clear renal carcinoma cell as the tumor antigen,and then making it to be modified by oxidized Mannan to acquire the tumor cell vaccine.Experimental groups include:blank group:DC?PBS group,control group:control?DC?786?0,experimental group:DC?Ox?Mannose?786?0 group. Taking the flow cytometry to detect the changes of DC phenotype,then taking the ELISA to detect the sencretion levels of supernatant of IL?12 of DC,then taking the CCK to detecte the cytotoxicity of lymphocytes(CTL)induced by DC of these experiment groups. Results Results by flow cytometry:the mature phenotype of DCs sensitized by Ox?Mannose?786?0 group included CD80,CD83,CD86 and HLA?DR expressed significantly higher than the other groups. As well as the secretion levels of IL?12. Meanwhile the cytotoxicity activity of lymphocytes(CTLs)induced by DCs which are sensitized by Ox?Mannose?786?0 increased more significantly than the other groups. Conclusion Glycosylated Antigens can be more effective in sensitizing antigen?presenting cells DC,and stimulating them to be maturation,while the killing effect to tumor cells also have noticeably improved.
