Isolation, culture and surface markers detection of human umbilical cord mesenchymal stem cells

VernacularTitle:人脐带间充质干细胞分离培养及表面标志检测
Author: Kai FENG ; Li XIAO ; Xihui MA ; Yu GAO ; Xiangrui KONG
Publication Type:Journal Article
Keywords: Fetal blood; Mesenchymal stem cells; Cell culture techniques; Antigens,surface; Wharton' s jelly
From: Journal of Leukemia & Lymphoma 2013;22(6):354-356
CountryChina
Language:Chinese
Abstract: Objective To investigate the methods of isolation and culture in vitro and detect the surface markers of human umbilical cord mesenchymal stem cells.Methods Human umbilical cord Wharton' s jelly was separated and cut up as small as possible,and then cultured with α-MEM.Human umbilical cord mesenchymal stem cells could be obtained by culturing the tissue block adhered the bottle wall.And the cells were passaged at a certain density.The surface markers of human umbilical cord mesenchymal stem cells were detected by FACS when the cells were in Generation Three.Results Human umbilical cord mesenchymal stem cells were obtained from Wharton' s jelly conveniently,with fibroblast shape and stable proliferation and passage.CD29,CD44,CD105 were strongly expressed on human umbilical cord mesenchymal stem cells.But CD45,CD34,HLA-DR,HLA-G,CD80,CDs6 were not expressed.Conclusion Human umbilical cord mesenchymal stem cells can be obtained effectively from the culture of the tissue block,which provides a rich source of cells for tissue engineering.