Role of PARP-2 in cardiac hypertrophy in rats
10.3969/j.issn.1000-4718.2015.07.001
- VernacularTitle:PARP-2在大鼠心肌肥大中的作用
- Author:
Guangyou ZHOU
;
Biao GENG
;
Tao GENG
;
Rufeng AN
;
Fang TIAN
;
Peiqing LIU
- Publication Type:Journal Article
- Keywords:
Poly( ADP-ribose) polymerase-2;
Angiotensin Ⅱ;
Abdominal aortic coarctation;
Cardiac hyper-trophy
- From:
Chinese Journal of Pathophysiology
2015;(7):1153-1159
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the expression of poly(ADP-ribose) polymerase-2 (PARP-2) during rat car-diac hypertrophy in vitro and in vivo, and to explore the effects of PARP-2 on the cardiac hypertrophy.METHODS:Ab-dominal aortic coarctation ( AAC) was performed to establish a model of pressure overload-induced cardiac hypertrophy in SD rats.The expression of PARP-2 at mRNA and protein levels in the myocardium was determined by real-time PCR and Western blot.The hypertrophy model of the cardiomyocytes was induced by treating the cells with angiotensinⅡ( AngⅡ) . PARP-2 was knocked down by siRNAs in neonatal rat cardiomyocytes and the cardiomyocyte hypertrophy was evaluated by measuring the mRNA levels of ANF, BNP, and β-MHC and the cellular surface area.RESULTS: The expression of PARP-2 at mRNA and protein levels was both increased in the AAC rats as compared with those in the sham animals.The expression of PARP-2 at mRNA and protein levels was also increased in a time-and concentration-dependent manner in AngⅡ-induced hypertrophy model of the cardiomyocytes.In the neonatal rat cardiomyocytes, knockdown of PARP-2 ex-pression by siRNA attenuated AngⅡ-induced cardiac hypertrophy of the cardiomyocytes, indicating that endogenous PARP-2 played a positive regulatory role in cardiac hypertrophy.CONCLUSION:The mRNA and protein levels of PARP-2 in-crease in the in vitro and in vivo models of cardiac hypertrophy.Knockdown of PARP-2 protects cardiomyocytes from hyper-trophy.
