Effect of decorin on proliferation and collagen type I synthesis of stiff knee joint synovial type B cells
10.3969/j.issn.1000-4718.2015.07.022
- VernacularTitle:Decorin对僵直膝关节滑膜B型细胞增殖及I型胶原蛋白表达的影响
- Author:
Bangrong XING
;
Yuxian CHEN
;
Hua ZENG
;
Xiaoyan YANG
;
Caiqian LIANG
;
Weixiong ZHOU
;
Qinglei KONG
;
Jianhua HAN
;
Yongbiao ZHANG
- Publication Type:Journal Article
- Keywords:
Knee stiffness;
synovial type B cells;
Decorin;
Collagen type Ⅰ
- From:
Chinese Journal of Pathophysiology
2015;(7):1277-1281
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effects of decorin on procollagen type I (PcI), mRNA expression,collagen type I synthesis and proliferation of synovial type B cells of stiff knee joint synovial membrane.METHODS:Type B cells of synovial membrane were isolated from the stiff knee joint synovial membrane and cultured in vitro.The cells were treated with decorin at concentrations of 0.1 mg/L, 5 mg/L and 10 mg/L.After cultured for 24 h, 48 h and 72 h, the cell proli-feration rates were measured by MTT colorimetric determination.Cell cycle distribution and apoptosis were analyzed by flow cytometry.The mRNA level of Pc I was detected by RT-PCR, while collagen type I was measured by Western blot.RE-SULTS:The proliferation of synovial type B cells was significantly inhibited, the percentage of synovial type B cells at G1 phase was significantly increased by 5 mg/L and 10 mg/L decorin (P<0.05), and PcⅠmRNA expression and collagen type I synthesis were significantly decreased.The cells with late apoptosis were not found in control group and experimental groups.CONCLUSION:Recombinant human decorin inhibits synovial type B cell proliferation and decreases PcⅠmRNA expression and collagen type I synthesis in synovial type B cells of stiff knee joint synovial membrane in vitro, suggesting that decorin potentially contributes to the therapy of human knee stiffness.
