Changes of large-conductance calcium-activated potassium channel in gastric smooth muscle cells of diabetic gastroparesis rats
10.3969/j.issn.1000-4718.2015.07.024
- VernacularTitle:糖尿病胃轻瘫大鼠胃平滑肌细胞BKCa通道的变化
- Author:
Jianhui XU
;
Xiaoyu HOU
;
Hongxian ZHAO
- Publication Type:Journal Article
- Keywords:
Diabetes gastroparesis;
Large-conductance calcium-activated potassium channel;
Gastric smooth muscle cells
- From:
Chinese Journal of Pathophysiology
2015;(7):1288-1293
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM: To discuss the relevance between the pathogenesis of diabetic gastroparesis and the large-conductance calcium-activated potassium channels ( BKCa ) in gastric smooth muscle cells.METHODS:The SD rats were randomly divided into control group and model group.The gastric smooth muscle cells of the SD rats were enzymatically iso-lated in a low calcium solution containing papain.The current was recorded by patch clamp single channel recording tech-nique.The expression of KCNMA and KCNMB1 were observed by the method of immunohistochemistry.RESULTS:The value of BKCa single channel conductance was (220.10 ±10.90) pS;the channels had distinct voltage dependent and cal-cium dependent characteristics.In outside-out patch (Vm =+30 mV), the activation of BKCa was blocked by 200 nmol/L IbTX completely.Compared with control group, the open probability and amplitude of current in model group significant-ly increased, while the mean open time and mean close time significantly decreased.Compared with control group, the ex-pression of KCNMB1 in model group was significantly increased.CONCLUSION: Up-regulation of β1-subunit and in-crease in BKCa functional activities may be associated with diabetes gastroparesis in rats.
