miR-205 inhibits invasion of glioma cells via targeting TBX18
10.3969/j.issn.1000-4718.2015.07.012
- VernacularTitle:miR-205通过靶向调控 TBX18抑制神经胶质瘤细胞的侵袭能力
- Author:
Guopei ZHENG
;
Xiaoting JIA
;
Cong PENG
;
Zhimin HE
- Publication Type:Journal Article
- Keywords:
Glioma;
MicroRNA-205;
Neoplasm invasion;
TBX18 protein
- From:
Chinese Journal of Pathophysiology
2015;(7):1219-1224
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To explore the expression pattern of microRNA-205 ( miR-205) in glioma tissues and its role in the invasion of glioma cells.METHODS:The expression of miR-205 and TBX18 was detected by real-time PCR and immunohistochemical observation, respectively.Transwell assay was used to examine the invasion change of U251 glioma cells after miR-205 overexpression via miR-205 mimics or decrease in miR-205 expression by miR-205 inhibitor.The target of miR-205 was searched by bioinformatics analysis combined with experimental analysis.The protein level of TBX18 was determined by Western blotting after siRNA transfection and Transwell assay was conducted.RESULTS:miR-205 expres-sion was downregulated in 82.6%of detected glioma tissues and TBX18 was significantly overexpressed in glioma tissues compared with normal tissues.miR-205 overexpression remarkably inhibited the invasion potential of U251 glioma cells with a decrease in the invasive cells (P<0.01), while inhibition of miR-205 significantly enhanced the invasion ability of U251 cells.Mechanically, miR-205 directly targeted TBX18 and downregulation of TBX18 also significantly inhibited the invasion potential of U251 cells with a decrease in the invasive cells ( P<0.01 ) .CONCLUSION: miR-205 expression is de-creased in glioma, and miR-205 inhibits glioma cell invasion via targeting TBX18.Our research contributes to the mecha-nisms responsible for glioma invasion and provides theoretical base for developing new therapeutic strategy to treat glioma.
