Panning and identification of antagonistic active peptides specifically bin-ding to the first and second extracellular membrane loops of rat CCR5 by technique of phage display peptide library
10.3969/j.issn.1000-4718.2015.07.013
- VernacularTitle:应用噬菌体展示肽库技术淘选大鼠 CCR5膜外第一、二胞外环特异性结合的活性拮抗肽与初步鉴定
- Author:
Sixue LIU
;
Mei HU
;
Xiaoyan YE
;
Huarong HUANG
;
Yingqiang ZHONG
- Publication Type:Journal Article
- Keywords:
CC chemokine receptor 5;
Antagonistic peptide;
Phage display
- From:
Chinese Journal of Pathophysiology
2015;(7):1225-1230
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM: To pan the active peptides which specifically bound to the first and second extracellular membrane loops of rat CC chemokine receptor 5 ( CCR5 ) .METHODS: The technique of phage display peptide library was used and binding ability of the peptides was identified.The amino acid sequences of the first and second extracellular loops of rat CCR5 were searched in the protein database and chemically synthesized corresponding linear peptides were used as targets in the biopanning.After 3 to 4 rounds of screening with Ph.D.TM-7 Phage Display Peptide Library were per-formed, the specific phages were collected and primarily identified by ELISA.RESULTS:The sequences of the peptides displayed on the selected phages were GHWKVWL and HYIDFRW, both of them exhibited positive in phage binding ELISA and the binding to phages and targets were concentration dependent and saturable.CONCLUSION:Two antagonis-tic active peptides specifically binding to CCR5 were successfully obtained by the technique of phage display peptide librar-y, and the binding ability to the first and second extracellular membrane loops of rat CCR5 were proved in vitro.
