Differences of genome-wide methylation level and methylated genes be-tween nasopharyngeal carcinoma cells in same genetic background but different radiation resistance
10.3969/j.issn.1000-4718.2015.07.026
- VernacularTitle:同源不同辐射抗拒 NPC 细胞间总甲基化水平及基因甲基化表达差异的探讨
- Author:
Haidan LUO
;
Donghui WANG
;
Weida LIN
;
Bojin SU
;
Cunyou ZHAO
;
Huiling YANG
- Publication Type:Journal Article
- Keywords:
Radiation resistant;
Methylation;
Nasopharyngeal carcinoma;
Gene element
- From:
Chinese Journal of Pathophysiology
2015;(7):1300-1304
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To compare the differences of the genome-wide methylation levels and methylated regions be-tween nasopharyngeal carcinoma ( NPC) cells in the same genetic background but different radiation resistance ( CNE-2 cells and CNE-2R cells).METHODS:Using the method which was developed by Doctor Zhao Cun-you, based on using methyl-sensitive restriction enzyme to measure the genome-wide methylation levels.In addition, MeDIP-Seq was used to analyze the methylated regions in 6 gene functional elements, including the upstream 2k sequence, 5’ UTR, coding se-quence, intron, 3’UTR and downstream 2k sequence, between CNE-2 cells and CNE-2R cells.RESULTS:The genome-wide methylation level was approximately 30%lower in CNE-2R cells than that in CNE-2 cells.No obvious difference on the amount of genes and the coverage of the peak in the 6 gene functional elements was observed.However, the methylation pattern of plentiful genes had altered in the gene function elements.CONCLUSION:The genome-wide methylation levels and methylated regions between NPC cells in the same genetic background but different radiation resistance were quite dif-ferent, indicating that the DNA methylation may be associated with NPC radioresistance.
