L-carnitine inhibits eryptosis induced by uremic serum
10.3969/j.issn.1000-4718.2015.07.031
- VernacularTitle:左卡尼汀抑制尿毒症血清诱导的红细胞衰亡
- Author:
Yun SUN
;
Gang LIU
;
Xuegang LI
;
Yimin SHI
;
Guangju GUAN
- Publication Type:Journal Article
- Keywords:
Uremic serum;
Eryptosis;
L-carnitine;
Oxidative stress
- From:
Chinese Journal of Pathophysiology
2015;(7):1324-1328
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM: To investigate whether L-carnitine ( LC) inhibits the eryptosis effect of uremic serum on erythrocytes.METHODS:Erythrocyte suspension (2%) was cultured and divided into 3 groups in vitro: control group ( C group) , uremic serum group ( U group, 30%uremic serum) , and uremic serum+LC group ( L group, 30%uremic serum+200 μmol/L LC) .Erythrocytes were collected at 24 h and 48 h.Eryptosis ( phosphatidylserine expression repre-sents eryptosis) was estimated by flow cytometry with Annexin V staining.The content of reactive oxygen species ( ROS) was also detected.Glutathione ( GSH) was measured by ELISA.RESULTS:Eryptosis in C group was increased as the in-cubating time extended.Eryptosis in U group was higher than that in C group, while that in L group was lower than that in U group.Meanwhile, ROS content was higher and GSH was lower in U group than those in C group.ROS content was low-er and GSH was higher in L group than those in C group.CONCLUSION:LC inhibits uremic serum-induced eryptosis by decreasing ROS and increasing GSH, thus attenuating oxidative stress.
