Effect of microgroove surface morphology on the adhesion and proliferation of human gingival fibroblasts
10.3969/j.issn.1001-3733.2015.04.014
- VernacularTitle:微沟槽形貌对人牙龈成纤维细胞黏附形态及增殖的影响
- Author:
Yingzhen LAI
;
Jiang CHEN
;
Shan LIN
- Publication Type:Journal Article
- Keywords:
Soft tissue;
Implant;
Microgroove;
Human gingival fibroblasts(HGFs);
Contact guidance;
Cell pro-liferation
- From:
Journal of Practical Stomatology
2015;(4):515-519
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of microgroove surface morphology on the adhesion and proliferation of the human gin-gival fibroblasts(HGFs).Methods:Microgroove surfaces of titanium were fabricated by photolithography with parallel grooves of 15,30 or 60 μm in width and 5 μm or 10 μm in depth.The groups of the samples were denoted as T15 /5,T15 /10,T30 /5,T30 /10,T60 /5 and T60 /10.Smooth titanium surface(T0)was used as the control.The surface topography was observed by enviroment SEM(ES-EM).HGFs were cultured on the topographically modified surfaces.Morphology was observed by SEM.Cell proliferation was examined by CCK-8 kit.Results:HGFs on the microgroove surfaces had “contact guidance”parallel to the microgrooves,whereas the cells on T0 were oriented randomly.Cell proliferation was promoted and kept for longer period on T60 /10 surface.Conclusion:Surfaces of mi-crogrooves with increasing groove width and depth may achieve “contact guidance”for HGFs and promote cell proliferation.
