Cytotoxic effects of the extractions of three gingival retraction cords with different extraction time
10.3969/j.issn.1001-3733.2015.04.020
- VernacularTitle:3种排龈线不同时间浸提液的细胞毒性研究
- Author:
Ying NI
;
Zhenwei QIU
;
Xia SUN
;
Min YANG
;
Meijing YANG
- Publication Type:Journal Article
- Keywords:
Human gingival fibroblasts;
Cell culture;
Cytotoxicity;
Gingival retraction cords;
DL-adrenaline HCL;
Aluminium sulphat;
Apoptosis
- From:
Journal of Practical Stomatology
2015;(4):547-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the toxic effect of 3 different gingival retraction cords.Methods:DMEMextraction of DL-adren-aline HCl,aluminium sulphate and non-drug retraction cords with the extraction time of 5,10,15 and 30 min were respectively pre-pared and were used to culture human gingival fibroblasts(HGFs)in vitro respectively.Cell proliferation was tested by MTT assay. Cell apoptosis was examined by Annexin/PI method.Results:The 3 gingival retraction cord extractions inhibited the roliferation,pro-moted the apoptosis of HGFs(P <0.05),the effects were related to the extraction time.Conclusion:The 3 retraction cords have time-dependant cytotoxity.
