The enhancement of GABAA-mediated currents by sufentanil in isolated dorsal root ganglion neurons in rats
10.3969/j.issn.1006-5725.2015.09.003
- VernacularTitle:舒芬太尼对大鼠DRG神经元GABAA激活电流的增强作用
- Author:
Luowa SHU
;
Xuezheng ZHANG
;
Chuxiong PAN
- Publication Type:Journal Article
- Keywords:
Sufentanil;
Dorsal root ganglion (DRG);
Gamma-aminobutyric acid (GABA);
Whole-cell patch-clamp
- From:
The Journal of Practical Medicine
2015;(9):1391-1394
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the modulatory effect of different dosage of sufentanil on I GABA in rat dorsal root ganglion neurons. Methods The rat root ganglion neurons were enzymatically dissociated. The whole-cell patch clamp techniquewas applied to record the effect of 0.02 , 0.1, 0.5, 2.5 μmol/L sufentanil and 0.5 mol/L sufentanil + 1 nmol/L Naloxone, a non-specific ityopioid receptor antagonist on IGABA. Results 0.02, 0.1, 0.5, 2.5 μmol/L sufentanil potentiated IGABA to (108.7 ± 6.7)%,(122.0 ± 2.3)%, (146.7 ± 7.9)% and (130.1 ± 5.6)%, respectively (n = 10; *P < 0.05, **P < 0.01). The potentiation role of 0.1,0.5, 2.5 μmol/L sufentanil on IGABA lasted 10 to 20 min The potentiation could be blocked by Naloxone (n = 7; *P < 0.05, **P < 0.01). Conclusion Sufentanil activates μ opioid receptor and potentiates the action of GABAA receptor, and the potentiation could be blocked by Naloxone. The enhancement of currents by sufentanil may increase GABA A receptor-mediated presynaptic inhibition at the spinal cord level.
