The effects of cadherin-11 transfection on the migration and differentiation of rat dental pulp cells
10.3969/j.issn.1001-3733.2015.04.04
- VernacularTitle:Cadherin-11基因转染对大鼠牙髓细胞迁移和分化的影响
- Author:
Chunlan GAN
;
Wan CHEN
;
Jie CAI
;
Wenqing ZHAO
;
Yu WU
- Publication Type:Journal Article
- Keywords:
Cadherin-11;
Rat dental pulp cells;
Migration;
Differentiation
- From:
Journal of Practical Stomatology
2015;(4):469-473
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the migration and odontogenesis of rat dental pulp cells transfected with adenovirus vector enco-ding cadherin-11.Methods:The dental pulp cells were cultured and transfected with pDC316-mCMV-EGFP-Cadherin-11.Cad-11 protein expression of the cells was examined by immunohistochemistry staining.The odontogenic differentiation of the cells was studied by alizarin red staining and ALP staining.The adhesion and migration of the cells were tested.Results:Transfection of pDC316-mC-MV-EGFP-Cadherin11 promoted Cad-11 protein expression and ALP activity,increased calcified nodule formation(P <0.05),adhe-sion and migration of rat dental pulp cells(P <0.05).Conclusion:Cadherin-11 may promote the odontoblast differentiation and mi-gration of rat dental pulp cells.
