Comparative study between mesenchymal stem cells derived form orofacial bone and those from long bone
10.3969/j.issn.1001-3733.2015.04.016
- VernacularTitle:颌骨和长骨骨髓间充质干细胞的比较研究
- Author:
Lin YUAN
;
Han WANG
;
Jin SUN
;
Zhengyi YANG
;
Lu HAO
;
Guangsi PAN
;
Yina CAO
;
Jun QIAN
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cells;
Orofacial bone;
Long bone;
Osteogenesis
- From:
Journal of Practical Stomatology
2015;(4):526-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the proliferation and osteogenic differentiation between human bone marrow mesenchymal stem cells from orofacial bone(OMMSCs)and those from long bone(BMMSCs).Methods:OMMSCs were isolated from orthognathic surgical sites and cultured by limited dilution.BMMSCs were obtained from bone marrow of volunteers and isolated by density gradient centrifugation method.The surface markers of the cells were detected by flowcytometry.Single-colony formation,CCK assay and cell circle analyses were conducted.Osteogenic differentiation ability was evaluated by ALP activity test and Alizarin red staining after osteogenic induction culture.Results:The cell surface markers STRO-1 and CD105 of both stem cells were positive,CD34,CD31 and CD45 were negative. OMMSCs generated significantly higher numbers of colonies than BMMSCs.In addition,OMMSCs had a higher proliferation rate and more cells in proliferative(S +G2 )stage than BMMSCs.After osteogenic induction for 3,5,7 and 10 d,OMMSCs showed higher levels of ALP activity.OMMSCs formed significantly more mineralized nodules than BMMSCs after 21-day ostogenic induction.Conclusion:The proliferation and osteogenic differentiation capacity of OMMSCs are higher than those of BMMSCs.
