Effects of methylated CpG islands in promoter region on the expression of KIR3DL1 protein
10.3760/cma.j.issn.0254-5101.2015.01.003
- VernacularTitle:KIR3 DL1启动子区域 CpG 岛甲基化对抗原表达的影响
- Author:
Sudan TAO
;
Yanmin HE
;
Lina DONG
;
Wei ZHANG
;
Ji HE
;
Faming ZHU
;
Hangjun LYU
- Publication Type:Journal Article
- Keywords:
Killer cell immunoglobulin like receptor;
Methylation;
Promoter
- From:
Chinese Journal of Microbiology and Immunology
2015;(1):14-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of methylated CpG islands in the promoter region on the expression of killer cell immunoglobulin-like receptor 3DL1 (KIR3DL1).Methods Three voluntary unpaid blood donors carrying high expression allele KIR 3DL1*01502 and three donors carrying low expres-sion allele KIR3DL1*005 were recruited in this study .The nucleotide sequences and the methylated CpG islands in the promoter regions of KIR 3DL1*01502 allele and KIR3DL1*005 allele were analyzed .The NK cells expressing KIR3DL1*01502 and KIR3DL1*005 were respectively treated with 5-aza for the dem-ethylation of CpG islands within the promoters .The expression of KIR3DL1 protein on the surface of NK cells was measured with flow cytometer .Results Two differences at nucleotide sites -65 and -269 were detected within the promoter regions of KIR3DL1*01502 and KIR3DL1*005, resulting in two distinct CpG islands.The CpG islands within the promoter of KIR 3DL1*01502 allele were highly methylated .The ex-pression of KIR3DL1 protein on NK cells which carried KIR 3DL1*01502 allele was significantly increased after the demethylation of CpG islands .However , the treatment of demethylation had no significant effects on the expression KIR3DL1 protein on NK cells harboring KIR3DL1*005 allele.Conclusion The methylated CpG islands within the promoter of KIR 3DL1*01502 allele affected the antigen expression on the surface of NK cells.Different KIR3DL1 alleles might show different mechanisms in regulating antigen expression .
