Preparation and performance detection of carbon fiber-polylactic acid-polyethylene glycol composite scaffold
10.3969/j.issn.2095-4344.2015.21.017
- VernacularTitle:碳纤维-聚乳酸-聚乙二醇复合支架的制备及性能检测
- Author:
Changyan ZHOU
;
Qinghuan ZHOU
;
Jing BIAN
;
Ke CHEN
;
Wen CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;19(21):3371-3376
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The mechanical performance and cytocompatibility of polylactic acid-polyethylene glycol (PLA-PEG) scaffold is poor via long-term experiments; therefore, other materials are added to the scaffold in order to improve its biological activity and mechanical properties. OBJECTIVE:To investigate the preparation process of carbon fiber (CF)-PLA-PEG scaffold, and to test its performance. METHODS:Modified CF-PLA-PEG composite scaffolds were prepared using solution injection-particle leaching method. Ultra-structure, porosity, water-absorbing quality, degradation rate and mechanical properties of CF-PLA-PEG composite scaffolds and PLA-PEG scaffolds were compared. Rat osteoblasts were co-cultured with these two kinds of scaffoldsin vitro, and the cel adhesion rate was detected by precipitation method after 12 hours. Cel counting was detected using MTT method to determine the cel proliferation at culture days 1, 3, 5, 7, 9. RESULTS AND CONCLUSION:The surface structure of PLA-PEG distributed uniformly, and the aperture was (404.0±10.5) μm. The carbon fiber surface of modified CF-PLA-PEG composite scaffolds had a lot of longitudinal grooves, the surface structure also distributed uniformly, and the aperture was (433.0±3.0) μm. Difference in scaffold aperture was of significance between the two groups (P < 0.05). The porosity, water-absorbing quality, elasticity modulus, compressive strength, degradation rate, cel adhesion rate and cel growth rate of CF-PLA-PEG were better than those of PLA-PEG (P < 0.05). These results show that the CF-PLA-PEG scaffolds have good mechanical property and cel compatibility.
