Effects of preincubation medium and other factors affecting in vitro fertilization rate of post-thawed genetically engineering mouse spermatozoa
10.3969/j.issn.1671.7856.2015.001.008
- VernacularTitle:获能培养液等因素对遗传工程小鼠冻融精子体外受精率的影响
- Author:
Qin ZUO
;
Tao FAN
;
Cuiping ZHANG
;
Wendong YANG
;
Jinsong WANG
;
Changfa FAN
;
Zuomin LIU
;
Zhengming HE
;
Baowen LI
- Publication Type:Journal Article
- Keywords:
Sperm;
Cryopreservation;
In vitro fertilization;
Embryo transfer;
Genetically engineering mice
- From:
Chinese Journal of Comparative Medicine
2015;(1):45-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect of in vitro fertilization ( IVF) and mouse sperm cryopreservation , to establish a simple and economic frozen system for the genetically engineering mice preservation .Methods Sperm from genetically engineering mice were cryopreserved , IVF was performed using post-thawed sperm, then embryo transfer, to compare the effects of cryopreservation medium、age of male mice and sperm preincubation medium .Results Using CPA as sperm cryopreservation medium , when PM was used thawed-sperm preincubation in IVF , the fertility rates were from 82.49%to 91.43%, when HTF was used thawed-sperm preincubation in IVF , the fertility rates were from14.46%to 27.38%, there was a signification difference between PM and HTF sperm preincubation medium;10 to 35 weeks male genetically engineering mice sperm were succeed cryopreservation , and positive mice were procreated after 2-cell embryos were transferred;R18S3、CPM and CPA was used to freeze sperm , the fertility rates were 75.85%、88.89%to 94.27%, positive mice were procreated after 2-cell embryos were transferred;2-cell embryos after IVF were freezed , then thawed and positive mice were procreated after 2-cell embryos were transferred .Conclusion Using CPA as sperm cryopreservation medium , when PM was used thawed-sperm preincubation in IVF , genetically engineering mice sperm were succeed cryopreservation .
