Development of Fast Atom Bombardment Mass Spectrometry for Determination of Antioxidant Activity of Phenolic Compounds
10.11895/j.issn.0253-3820.140452
- VernacularTitle:快原子轰击离子化质谱研究酚类化合物抗氧化作用
- Author:
Fen YANG
;
Jiuming HE
;
Ruiping ZHANG
;
Abliz ZEPER
- Publication Type:Journal Article
- Keywords:
Antioxidant activity;
Fast atom bombardment mass spectrometry;
Molecular ion peak;
Phenolic compound;
Flavonoids
- From:
Chinese Journal of Analytical Chemistry
2015;(2):233-238
- CountryChina
- Language:Chinese
-
Abstract:
A fast atom bombardment mass spectrometric method to predict and detect the antioxidant ability of phenolic compounds was developed to accelerate the pace of finding the antioxidant with higher effect and low toxicity. The effect of experimental conditions on the relative peak intensity ratio of M+· ion to [ M+H]+ion in the FAB mass spectra of the compound was investigated, including matrix, scan time and concentration. The correlation of antioxidant activity with the I ( M+· )/I ( [ M+H ]+) value of flavonoids obtained in FAB mass spectra was studied. Then the antioxidant activity of 12 phenolic compounds was predicted using the above method and the results were compared with those obtained from thiobarbituric acid ( TBA) method. The results show that the I( M+· )/I( [ M+H]+) value of the phenolic compound obtained from FAB mass spectra could reflect their antioxidant activity, which could help to accelerate the development of the antioxidant drug.
