Transforming growth factor αpromotes the proliferation, migration and adhesion of human endothelial progenitor cells
10.3969/j.issn.1000-4718.2015.01.007
- VernacularTitle:转化生长因子α促进人内皮祖细胞增殖、迁移和黏附
- Author:
Wenjing DAI
;
Jun ZHANG
;
Jingqun ZHOU
;
Changqing XIANG
;
Gang WANG
- Publication Type:Journal Article
- Keywords:
Transforming growth factor α;
Human endothelial progenitor cells;
Cell proliferation;
Cell migra-tion;
Cell adhesion;
Epidermal growth factor receptor
- From:
Chinese Journal of Pathophysiology
2015;(1):33-39
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effects of transforming growth factor-α( TGF-α) in the monoclonal formation , proliferation, migration and adhesiveness of human endothelial progenitor cells ( EPCs).METHODS: The isolated and cultured EPCs were treated with various concentrations of TGF-α(final concentrations of 1, 5, 10μg/L, respectively).At the same time, the PBS control and epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) group (10μg/L TGF-αplus 1∶1 000 EGFR-TKI) were set.The effects of TGF-αon monoclonal formation , proliferation, migration and adhesiveness of EPCs were determined by clone formation experiment , thiazolyl blue tetrazolium bromide (MTT), EdU, Transwell and adhesion assays , respectively.The expression of epithelial growth receptor (EGFR) and vascular endothelial growth factor ( VEGF) were measured by Western blotting .RESULTS:Different concentrations of TGF-αall significantly induced the monoclonal formation , proliferation, migration and adhesiveness of EPCs (P<0.01), which were inhibited by EGFR-TKI.The results of Western blotting showed that TGF-αalso induced the expression of EGFR and VEGF with a cer-tain concentration effect ( P<0.01) .CONCLUSION:By combining with EGFR induced the expression of VEGF , TGF-αsignificantly promotes the related cell function of monoclonal formation , proliferation, migration, adhesiveness in EPCs.
