Effect of drug-containing serum of Fushen Jiangzhuo formula on anti-fi-brosis factors HGF and BMP-7 in rat renal interstitial fibroblasts with re-nal tubulointerstitial lesion
10.3969/j.issn.1000-4718.2015.01.015
- VernacularTitle:扶肾降浊方含药血清对肾小管间质损害大鼠成纤维细胞抗纤维化因子 HGF 和 BMP-7的影响
- Author:
Chunyu LI
;
Xiaolu WEI
;
Weilian SU
;
Guoxia LI
- Publication Type:Journal Article
- Keywords:
Fushen Jiangzhuo formula;
Renal interstitial fibroblasts;
Serologic pharmacological test;
Hepato-cyte growth factor;
Bone morphogenetic protein-7
- From:
Chinese Journal of Pathophysiology
2015;(1):76-80
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the protective effects and mechanism of Fushen Jiangzhuo formula (FSJZ) on the rat renal interstitial fibroblasts with renal tubulointerstitial lesion .METHODS:The serum containing FSJZ and blank con-trol serum were prepared .The rat model of mesangial proliferative glomerulonephritis ( MsPGN) was established and ex-tended the time of modeling to 20th weeks for developing tubulointerstitial lesion naturally .The rat renal interstitial fibro-blasts at the end of 12, 16, 20 week of modeling were isolated and cultured .The effects of FSJZ on the expression of hepa-tocyte growth factor (HGF) and bone morphogenetic protein-7 (BMP-7) at mRNA and protein levels in the pathological re-nal interstitial fibroblasts were determined .RESULTS:The anti-fibrosis factors HGF and BMP-7 were changed in patho-logical renal interstitial fibroblasts .Renal tubulointerstitial lesion significantly down-regulated the expression of HGF and BMP-7, while the drug containing serum of FSJZ significantly up-regulated the expression of HGF and BMP-7.CONCLU-SION:Drug containing serum of FSJZ has protective effect on pathological renal interstitial fibroblasts by regulating the mRNA and protein expression of HGF and BMP-7.
