Hypoxia promotes apoptosis of neural stem cells and down-regulates miR-26 a
10.3969/j.issn.1000-4718.2015.01.016
- VernacularTitle:低氧诱导神经干细胞凋亡和 miR-26a 低表达
- Author:
Fang LI
;
Hongyan WEI
;
Yubin DENG
;
Xin LI
;
Hengjie LI
;
Chunlin HU
;
Yuanzheng LU
;
Xiaoxing LIAO
- Publication Type:Journal Article
- Keywords:
Cobalt chloride;
Apoptosis;
Neural stem cells;
miR-26a
- From:
Chinese Journal of Pathophysiology
2015;(1):81-86
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of cobalt chloride (CoCl2) on the apoptosis of neural stem cells (NSCs) and the expression of microRNA-26a (miR-26a) in vitro, and to explore the mechanisms of NSC apoptosis in-duced by CoCl 2 .METHODS:NSCs were exposed to CoCl 2 at different doses (200~600μmol/L) for 24 h.The cell via-bility and apoptosis were measured by CCK-8 assay and TUNEL method.The expression of miR-26a-3p, miR-26a-5p, GSK-3β, caspase-3, Bcl-2 and Bax was examined by real-time PCR.The protein levels of Bcl-2 and Bax were detected by Western blotting .RESULTS: The cell viability was inhibited and the apoptosis of NSCs was increased significantly by CoCl2 in a dose-dependent manner (P<0.05).CoCl2 at concentration of 400μmol/L for 24 h was used to induce apopto-sis and the expression of miR-26a was down-regulated compared with control (P<0.05).Exposure to CoCl2 at concentra-tion of 400μmol/L up-regulated the expression of GSK-3β, caspase-3 and Bax , down-regulated the expression of Bcl-2 and Bcl-2/Bax (P<0.05).CONCLUSION:CoCl2 at concentration of 400μmol/L induces the apoptosis of NSCs obviously . CoCl2 may induce the NSC apoptosis by mitochondrial apoptotic pathway .Declining miR-26a may be related to NSC apopto-sis.
