Treatment of Helicobacter pylori-infected gastritis in BALB/c mice by HP1188-IgY
10.3969/j.issn.1000-4718.2015.01.028
- VernacularTitle:HP1188-IgY 对感染幽门螺杆菌BALB／c 小鼠的治疗作用
- Author:
Fei HAN
;
Zhibang YANG
;
Jianying LI
;
Zheng ZHOU
;
Fangyi PENG
;
Hairong JIANG
;
Hongxin DU
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
HP1188-immunoglobulin yolk;
Sucralfate
- From:
Chinese Journal of Pathophysiology
2015;(1):148-153
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To evaluate the effects of treatment with HP 1188-immunoglobulin yolk ( HP1188-IgY) on Helicobacter pylori ( H.pylori)-infected gastritis in BALB/c mice.METHODS:BALB/c mice were used to establish an animal model of H.pylori-infected gastritis, and the mice were divided into 8 groups (10 mice per group).Oral antibiotics were used in group 1, 1 mg HP1188-IgY in group 2, 1 mg HP1188-IgY plus 30%sucralfate in group 3, 5 mg HP1188-IgY in group 4, 5 mg HP1188-IgY plus 30%sucralfate in group 5, PBS in group 6, and 30% sucralfate in group 7 with the treatment once per day for 10 d;and 2.5 mg HP1188-IgY was injected hypodermically twice with a 48-h interval in group 8.Another 10 mice were used as normal control in group 9.The planting of bacteria in the stomach was assayed by bacteri-al culture, rapid urease test, PCR and pathological sectioning .RESULTS:Intragastric administration with 1 mg HP1188-IgY plus 30%sucralfate per day effectively cured the injury of gastric mucosa caused by H.pylori infection, and the effect has no significant difference compared with antibiotics (P>0.05).CONCLUSION:We establish a BALB/c mouse mod-el infected with H.pylori successfully.Sucralfate (30%) is an ideal protectant for HP1188-IgY, which might decrease H. pylori infection in the stomach of BALB/c mice by oral inoculation .
