Level and clinical significance of RNA oxidative damage in human gastric cancer and para-carcinoma tissues
10.3969/j.issn.1000-4718.2015.01.032
- VernacularTitle:人胃癌及癌旁组织 RNA 氧化损伤水平的比较及临床意义
- Author:
Xianhui LIN
;
Junhua YU
;
Haihong LIN
;
Yangyang TU
;
Shunbin LUO
;
Tao XU
;
Tong CHEN
;
Xingcheng LIN
;
Sunzhi YE
;
Zhiqiang ZHENG
;
Jianping CAI
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Immunohistochemistry;
8-oxoguanosine;
LC-MS/MS
- From:
Chinese Journal of Pathophysiology
2015;(1):172-176
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the RNA oxidative damage in human gastric cancer tissue and para-carcinoma tissue for exploring the role of RNA oxidation in the occurrence of gastric cancer .METHODS:Immunohistochemical ob-servation and LC-MS/MS analysis were performed in 61 cases of gastric carcinoma .The position and concentration of 8-oxoguanosine ( 8-oxoGsn ) were detected , respectively . RESULTS: The results of immunohistochemical observation showed that 8-oxoGsn was obviously up-regulated in the gastric cancer .The positive staining mainly accumulated in the cy-toplasm of the tumor cells .The results of mass spectrometry showed that the level of 8-oxoGsn in the gastric cancer tissues was higher than that in the para-carcinoma tissues (P<0.05).CONCLUSION:8-oxoGsn is up-regulated in gastric canc-er.RNA oxidative damage may play important roles in the occurrence of gastric cancer .
