Comparison of 2 methods for inducing iPSC to differentiate into neural stem cells
10.3969/j.issn.1000-4718.2015.01.035
- VernacularTitle:2种诱导 iPSC 向神经干细胞分化方法的比较
- Author:
Tan YANG
;
Hua LIU
;
Yunshan WANG
- Publication Type:Journal Article
- Keywords:
Induced pluripotent stem cells;
Differentiation;
Neural stem cells
- From:
Chinese Journal of Pathophysiology
2015;(1):188-192
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To select an efficient way of promoting induced pluripotent stem cells ( iPSC) to differentiate into neural stem cells (NSC) by comparing 2 methods.METHODS:The culture system in method A contained SB431542 (5 mmol/L) and drosomophorin (5 mmol/L) with 100%initial cell density, while that in method B contained SB431542 (5 mmol/L) and drosomophorin (1 mmol/L) with 30%~50% initial cell density.For comparison and identification of the 2 methods, the growth state was observed under microscope , and the expression of Pax6, nestin, Sox1 and Sox2 was quantitatively detected by real-time PCR and flow cytometry .The related protein expression and the ability of spontaneous differentiation were determined by immunofluorescence analysis .RESULTS: The cells derived from method A with 5 mmol/L of SB431542 and drosomophorin and 100% initial cell density achieved the higher expression of Pax 6, nestin, Sox1 and Sox2.The growth state was better and the cells differentiated into neurons and astrocytes normally .CONCLU-SION:The method A was superior to method B , and we recommend the method A with 5 mmol/L of SB431542 and droso-mophorin and 100%initial cell density as the method for differentiating NSC .
