Difference expression of Cx37 and Cx40 of arteries in spontaneously hypertensive rats
10.3969/j.issn.1671-8348.2015.01.002
- VernacularTitle:Cx37和Cx40在自发性高血压大鼠不同动脉间的表达差异
- Author:
Lijun PAN
;
Xinzhi LI
;
Hongyu ZHUO
;
Li LI
;
Lili WEI
;
Xiushi YU
;
Junqiang SI
;
Ketao MA
- Publication Type:Journal Article
- Keywords:
rats,inbreb SHR;
mesenteric artery;
aorta;
electrical synapses;
connexin
- From:
Chongqing Medicine
2015;(1):4-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of connexin (Cx) in mesenteric artery (MA) and aorta of spontaneously hyper‐tensive rats (SHR) and normotensive rats .Methods Quantitative RT‐PCR and Werstern blot technique were used to compare the difference in the expression of Cx37 and Cx40 mRNA and protein in MA and aorta of SHR and normotensive rats .Results The level of Cx37 mRNA expression in MA from SHR was decreased compared with that of the normotensive rats (P<0 .05) ,and more significantly in aorta (P<0 .01) .Cx40 mRNA expression in MA from SHR was significantly decreased compared with that of the normotensive rats (P<0 .01) ,but no change in aorta (P>0 .05) .The expression of Cx37 protein in MA from SHR was decreased compared with that of the normotensive rats (P<0 .05) ,and more significantly in aorta (P<0 .01) .Cx40 protein expression in MA from SHR was significantly decreased compared with that of the normotensive rats (P<0 .05) ,but no change in aorta (P>0 .05) . Conclusion Hypertension may could decrease gap junctional communication in cells of MA and aorta from SHR by the downregu‐lation of the expression of Cx37 and/or Cx40 .
