Interventional effects of tongxinluo combined with atorvastatin and aspi-rin on adventitial inflammation in early stage of atherosclerosis
10.3969/j.issn.1000-4718.2015.01.029
- VernacularTitle:通心络联合阿托伐他汀、阿司匹林对家兔动脉粥样硬化早期血管外膜炎症因子表达的干预
- Author:
Yansong LANG
;
Hongying MI
;
Meizhi LIU
;
Guoqiang YUAN
- Publication Type:Journal Article
- Keywords:
Adventitia;
Inflammation;
Atherosclerosis;
Monocyte chemoattractant protein 1;
Interleukin-8;
Interleukin-10
- From:
Chinese Journal of Pathophysiology
2015;(1):154-159
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of a treatment proposal , which consisted of tongxinluo , atorvastatin and aspirin, on adventitial inflammation of early atherosclerosis in rabbit carotid artery .METHODS: The atherosclerotic model was established in the rabbits with silicone collar , which was positioned around the carotid arterial adventitia +high-cholesterol diet .New Zealand rabbits ( n =72 ) were randomly divided into 6 groups ( n=12 ): control group , model group, tongxinluo group, atorvastatin group, aspirin group, and three-drug combination group.The rabbits in control group were fed with common foodstuffs , and the rabbits in all the other groups were fixed the right carotid arteries with the silicone tube, and were fed with fatty foodstuffs .The rabbits in tongxinluo group , atorvastatin group and aspirin group were given the suspension of tongxinluo supermicropowder (0.3 g· kg-1· d-1), atorvastatin (2.5 mg· kg-1· d-1) and aspirin (12 mg· kg-1· d-1) respectively,and the rabbits in three-drug combination group were given the suspension of tongxinluo su-permicropowder (0.3 g· kg-1· d-1), atorvastatin (2.5 mg· kg-1· d-1) and aspirin (12 mg· kg -1· d-1) together. The rabbits in each group were fed with the corresponding medicines for 4 weeks .The tissue slices of carotid artery were observed under light microscope with HE staining .The change of blood lipid was detected by biochemical assay .The pro-tein levels of MCP-1, IL-1βand IL-10 in the carotid arterial adventitia were detected by ELISA .The immunohistochemical staining was used to detect the protein expression of IL-8 around the carotid arterial adventitia .RESULTS:Compared with control group, the contents of TC, TG and LDL-C were significantly increased , and the content of IL-10 was significantly decreased in model group .The levels of TC , TG and LDL-C were significantly decreased in tongxinluo group and atorvasta-tin group compared with model group , no significant difference between tongxinluo group and atorvastatin group was ob -served.In the three-drug combination group, the levels of TC, TG and LDL-C were lower than those in atorvastatin group and tongxinluo group .Compared with control group , the contents of MCP-1 and IL-1βwere significantly increased , and the content of IL-10 was significantly decreased in model group .Compared with model group , the contents of MCP-1 and IL-1βwere decreased in tongxinluo group , atorvastatin group and aspirin group , no significant difference between the 3 groups was observed.The content of IL-10 was decreased in three-drug combination group , and the contents of TC , TG and LDL-C were lower than those in tongxinluo group , atorvastatin group and aspirin group .The content of IL-8 was decreased in tongxinluo group , atorvastatin group , aspirin group and three-drug combination group .CONCLUSION: The strategy of three-drug combination enhances the effect of regulating the lipid metabolism and inhibiting the adventitia inflammation .It plays an important role to intervene in the process of atherosclerosis .
