Electroacupuncture on serum interleukin level in rat models of cerebral ischemia-reperfusion injury
10.1007/s11726-015-0815-0
- VernacularTitle:电针对脑缺血再灌注模型大鼠血清白介素的影响
- Author:
Ping WANG
;
Yanyun MU
;
Jie CHENG
;
Jie SHEN
;
Meihong SHEN
;
Xia CHEN
;
Qian LI
;
Yong SUN
;
Meirong GONG
- Publication Type:Journal Article
- Keywords:
Acupuncture Therapy;
Electroacupuncture;
Brain Ischemia;
Reperfusion Injury;
Interleukin-6;
Interleukin-8;
Interleukin-10;
Rats
- From:
Journal of Acupuncture and Tuina Science
2015;(1):9-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of electroacupuncture (EA) on serum interleukin (IL)-6, IL-8 and IL-10 in rat models of cerebral ischemia-reperfusion, and to discover the mechanism of EA in preventing and treating cerebral ischemia.
Methods:Male Sprague Dawley (SD) rats were randomized into a sham-operation (SO) group, a model control (MC) group, and an EA group, which were sub-grouped into a 6-hour group and a 24-hour group. In the SO group, rats only received vessel separation with filament placed inside without any treatment. In the MC and EA groups, the focal cerebral ischemia-reperfusion model was induced by using modified Longa method with intraluminal filament. The MC group didn’t receive any treatment;the EA group received EA at Baihui (GV 20) and Dazhui (GV 14) with sparse-dense wave for 30 min. The levels of serum IL-6, IL-8 and IL-10 were detected by using Elisa test.
Results: Six hours after ischemia-reperfusion injury, the levels of serum IL-6, IL-8 and IL-10 in the MC group were significantly higher than those in the SO group (P<0.01, P<0.05, P<0.05);the level of serum IL-8 in the EA group was significantly lower than that in the MC group (P<0.05), while there were no significant differences in comparing IL-6 and IL-10 between the EA group and the MC group. Twenty-four hours after ischemia-reperfusion injury, the levels of serum IL-6 and IL-8 in the EA group were significantly lower than those in the MC group (both P<0.05), while there were no significant differences in comparing the level of IL-10 among the three groups.
Conclusion:Early intervention by EA can regulate the levels of serum IL-6 and IL-8 in cerebral ischemic injury.
