Effect of Cold Self-blood Cardioplegia With Ulinastatin on Immature Myocardial Cell Apoptosis in Infant Patients
10.3969/j.issn.1000-3614.2014.12.013
- VernacularTitle:含乌司他丁自体冷血心脏停搏液对未成熟心肌细胞凋亡的影响
- Author:
Baoying MENG
;
Qing ZHANG
;
Xiaolan PAN
;
Yuanxiang WANG
;
Dingrong SHEN
;
Yunxing TI
;
Chao MA
;
Le PENG
- Publication Type:Journal Article
- Keywords:
Congenital heart disease;
Ulinastatin;
Self-blood Cardioplegia;
Apoptosis
- From:
Chinese Circulation Journal
2014;(12):1011-1014
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of cold self-blood cardioplegia with ulinastatin on immature myocardial cell apoptosis and protein expressions of Bcl-2, Bax in ventricular septal defect (VSD) infants.
Methods: A total of 60 infants received VSD repairing operation with cardiopulmonary bypass (CPB) in our hospital were summarized. The patients were randomly divided into 2 groups:Test group, the infants received cold self-blood cardioplegia with ulinastatin when aortic cross-clamp was closed. Control group, the infants received cold self-blood cardioplegia when aortic cross-clamp was closed. n=30 in each group. The right atrium tissue was collected before CPB and 10 min after releasing aortic cross-clamp. The index of myocardial cell apoptosis was observed by TUNEL method, and the protein expressions of Bcl-2, Bax were examined by immunohistological method.
Results: Both groups showed the higher index of myocardial cell apoptosis at 10 min after releasing aortic cross-clamp than 5 min before CPB, and the apoptosis index in Test group was lower than that in Control group, all P<0.05. The protein expressions of Bcl-2 and Bax were obviously increased at 10 min after releasing aortic cross-clamp than 5 min before CPB in both groups. Compared with Control group, Test group presented the higher Bcl-2 protein expression and lower Bax protein expression, all P<0.05.
Conclusion: Cold self-blood cardioplegia with ulinastatin could protect immature myocardum from ischemia-reperfusion injury in VSD infants during CPB operation in clinical practice.
