Effect of Low-Level Laser Irradiation Preconditioning on Milieu of Infarcted Myocardium in Experimental Rats
10.3969/j.issn.1000-3614.2015.01.013
- VernacularTitle:低能激光照射对梗死后心肌微环境影响的实验研究
- Author:
Jianfeng HOU
;
Xin YUAN
;
Hao ZHANG
;
Zhe ZHENG
;
Shengshou HU
- Publication Type:Journal Article
- Keywords:
Low-level laser irradiation;
Myocardial infarction;
Milieu
- From:
Chinese Circulation Journal
2015;(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of low-level laser irradiation (LLLI) preconditioning on milieu of infarcted myocardium in experimental rats.
Methods: The myocardial infarction (MI) model was established by left anterior descending (LAD) artery ligation in female rats. 3 weeks later, the qualified MI rats were randomly divided for 3 groups: ① LLLI preconditioning group, the rats received thoracotomy for LLLI by a 635nm, 5mW diode laser with the energy density of 0.96 J/cm2 for 150 seconds, n=26. ② Control group, the rats received thoracotomy for daylight irradiation, n=27. ③ Sham operation group, the rats received thoracotomy without LAD ligation, n=24. The Expressions of myocardial vascular endothelial growth factor (VEGF), glucose-regulated protein 78 (GRP78), superoxide dismutase (SOD) and malondialdehyde (MDA) were evaluate by real time-PCR, Western blot analysis and other relevant laboratory test at 1 hour, 1 day and 1 week after treatment. The myocardial cell apoptosis was examined by TUNEL staining, and left ventricular function was detected by echocardiography.
Results: LLLI preconditioning obviously increased the myocardial VEGF, GRP78 expression and SOD activity, decreased MDA production; while it could not really improve the myocardial cell apoptosis at peri-infarcted area and left ventricular function in experimental rats.
Conclusion: LLLI preconditioning may improve the milieu of infarcted myocardium via decreasing the oxidative stress in experimental rats.
