Study on the Expression of mRNA of Tumor Necrosis Factor-alpha and Interleukin-6 in the Cell Lines of Squamous Cell Carcinoma.
- Author:
Jin Su AHN
1
;
Kyung Wook KIM
;
Jae Hoon LEE
Author Information
1. Department of Oral & Maxillofacial Surgery, College of Dentistry, Dankook University.
- Publication Type:Original Article
- MeSH:
Actins;
Carcinoma, Squamous Cell*;
Cell Line*;
Cell Line, Tumor;
Epithelial Cells;
Interleukin-6*;
Mouth Neoplasms;
Polymerase Chain Reaction;
Reverse Transcriptase Polymerase Chain Reaction;
RNA;
RNA, Messenger*;
Tumor Necrosis Factor-alpha*;
Ultraviolet Rays;
X-Ray Film
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2001;27(6):535-542
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of this study was to examine the mRNA levels of TNF-alpha and IL-6 in the cell lines of normal oral keratocyte and oral squamous cell carcinoma. Total RNA was extracted from these cell lines, observed under UV light, developed by radiographic films of PCR products via reverse transcriptase polymerase chain reaction (RT-PCR) amplication, and measured with densitometer. Each mRNA level of these cell lines divided by beta-actin mRNA level was compared to that of normal control group. The results were as follows : 1. Higher mRNA expression of TNF-alpha than IL-6 in the normal oral epithelial cell line. 2. In general, expression of mRNA of IL-6 appeared 3-4 times more in tumor cell lines than in control group. 3. mRNA expression of TNF-alpha showed variable expression in tumor cell lines, unlike normal cell line. 4. There are no special connections between differentiation of oral cancer cell lines and mRNA expression of TNF-alpha and IL-6. From the above results, expression of mRNA of IL-6 in the cell lines of squamous cell carcinoma used in this study has higher than the normal oral epithelial cell line, but there are no relationship between the differentiation of oral cancer cell lines and the expression of mRNA of TNF-alpha and IL-6.
