Effect of angiotensinⅡon expression of Notch pathway and Nephrin in podocyte
10.3969/j.issn.1001-1978.2015.02.020
- VernacularTitle:血管紧张素Ⅱ对足细胞Notch通路及Nephrin表达的影响
- Author:
Feng GAO
;
Tao ZHANG
;
Xiaomei WANG
;
Yufeng ZHAO
;
Shuxia LIU
- Publication Type:Journal Article
- Keywords:
angiotensin Ⅱ;
podocyte;
Notch path-way;
Nephrin;
angiotensin Ⅱ type 1 receptor antago-nism;
valsartan
- From:
Chinese Pharmacological Bulletin
2015;(2):247-250
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the expression of Notch pathway and Nephrin in angiotensin Ⅱ ( AngⅡ)-stimulated mice podocyte. Methods Mice podo-cyte was stimulated by AngⅡ, and then was treated with valsartan. The levels of Notch1, Notch intracellu-lar domain 1 ( NICD1 ) , Hes1 and Nephrin were deter-mined by immunofluorescence, Western blot and Real-time PCR. Results AngⅡincreased Notch1, NICD1 and Hes1 expression, and decreased Nephrin expres-sion in a time-dependent manner ( P<0. 01 ) . Valsar-tan inhibited AngⅡ-induced activation of Notch path-way and enhanced Nephrin level ( P <0. 01 ) . Con-clusion AngⅡdecreases Nephrin expression in podo-cyte by activating Notch pathway.
