Hinokitiol induces clear cell renal cancer 786-O cell apoptosis via autophagy induction
10.3969/j.issn.1000-8179.20141412
- VernacularTitle:桧木醇诱导人肾透明细胞癌786-O细胞凋亡及其机制研究
- Author:
Xiaochen NI
;
Zhihong ZHAO
;
Yongliang MA
;
Zongtao REN
;
Bin LIU
;
Bo FAN
;
Shufei WEI
;
Aili ZHANG
- Publication Type:Journal Article
- Keywords:
hinokitiol;
renal cancer;
apoptosis;
proliferation;
autophagy
- From:
Chinese Journal of Clinical Oncology
2015;(1):43-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of hinokitiol on the proliferative inhibition and apoptosis induction in human clear cell renal cancer 786-O cells. Methods:CCK-8 assays were performed to analyze the effects of hinokitiol on the proliferation of 786-O cells. The apoptosis rate was determined by flow cytometry. EGFP-LC3 microscopy assays were performed to assess the autoph-agy flux. Cleaved Caspase-3, LC3, and P62 were detected by Western blot. Results: Hinokitiol could inhibit the proliferation of the 786-O cells and could induce cell apoptosis via Caspase pathway. Hinokitiol induced the autophagy of 786-O cells, increased LC3 ex-pression, and downregulated P62 expression. Conclusion: Hinokitiol can inhibit the proliferation of 786-O cells and can induce cell apoptosis via autophagy induction.
