Effects of Mieyou Decoction on TLR2 and TLR4 in H. pylori-associated Gastritis Mice of
10.3969/j.issn.1005-5304.2015.02.018
- VernacularTitle:灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证模型小鼠Toll样受体2、4的影响
- Author:
Yan LUO
;
Bin YU
;
Yin XU
;
Xuan GUO
;
Zhonghua DU
;
Jiao YIN
;
Rong XIA
- Publication Type:Journal Article
- Keywords:
Mieyou Decoction;
Hp-associated gastritis;
spleen-stomach damp-heat syndrome;
TLR2;
TLR4mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(2):64-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of Mieyou Decoction in the treatment of Hp-associated gastritis of spleen-stomach damp-heat syndrome. Methods Seventy BALB/c mice were randomly divided into control group, model group, high concentration of Mieyou Decoction group, low concentrations of Mieyou Decoction group, and gastric triad group, with 14 rats in each group. BALB/c mice of Hp-associated gastritis of spleen-stomach damp-heat syndrome models were established by composite factor. After the success of modeling and continuous 14-day administration, the expressions of TLR2, TLR4 protein, TLR2 mRNA, and TLR4 mRNA were detected by immunohistochemistry and qPCR. ELISA was used to detect the expressions of IL-6 and IL-8 in the serum. Results Compared with control group, the expressions of TLR2, TLR4 protein, mRNA, IL-6, and IL-8 in model group significantly increased (P<0.01);the expressions of TLR2, TLR4 protein, mRNA, IL-6, and IL-8 in high concentration of Mieyou Decoction group, low concentrations of Mieyou Decoction group, gastric triad group were lower than model group, with statistical significance (P<0.01);the expressions of TLR2, TLR4 protein, mRNA, IL-6, and IL-8 in high concentration of Mieyou Decoction group were higher than gastric triad group, but the differences were not statistically significant (P>0.05). Conclusion Mieyou Decoction may play a role in the treatment of Hp-associated gastritis of spleen-stomach damp-heat syndrome through intervention in the expressions of TLR2 and TLR4.
