Evaluation of Quality Coherence of Angelicae Dahuricae Radix (Baizhi) Decoction Pieces Based on HPLC Fingerprint
10.3969/j.issn.1005-5304.2015.02.021
- VernacularTitle:HPLC指纹法评价白芷饮片质量的一致性
- Author:
Xiaoyi WU
;
Zhimao CHAO
;
Chun WANG
;
Li YU
;
Yaohua LIANG
;
Wen SUN
;
Yonghong LIAO
- Publication Type:Journal Article
- Keywords:
Angelicae Dahuricae Radix;
fingerprint;
decoction piece;
imperatorin;
isoimperatorin;
quality coherence
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(2):74-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the quality coherence of commercial Angelicae Dahuricae Radix (Baizhi) decoction pieces. Methods The samples of 12 batches of commercial decoction pieces, 1 batch of sulphur fumigated Baizhi, and 1 batch of naturally dried Baizhi were collected. HPLC method was used to determine the fingerprints and the contents of imperatorin and isoimperatorin by C18 column (4.6 mm×250 mm, 5 μm) with a gradient mobile phase of acetonitrile-water solution system at the flow rate of 1.0 mL/min. The column temperature was set at 35 ℃, and the max plot of detection wavelength was in 210-800 nm. Similarity calculation was used to analyze the data. Results The HPLC fingerprint analysis method was established with 12 common peaks. The similarities of 12 batches of commercial decoction pieces of Baizhi were 0.840-0.973. Their similarities compared with the sulphur fumigated Baizhi were 0.672-0.908. Compared with naturally dried Baizhi, the similarities of fingerprint were 0.536-0.684. The contents of imperatorin and isoimperatorin in 12 batches of commercial decoction pieces were 0.035%-0.140% and 0.028%-0.069%, respectively. Conclusion The quality of Baizhi decoction pieces was consistent. It can be speculated that Baizhi decoction pieces were processed with sulphur fumigation.
