Analysis of Genetic Relationship of Astragalus membranaceus and Codonopsis pilosula from Different Cultivars (Varieties) by ISSR

VernacularTitle:不同黄芪和党参栽培品种（系）遗传关系的ISSR分析
Author: Tiantian ZHU ; Ling JIN ; Xiaorui LIU ; Tao DU ; Yingdong LI ; Peisi ZHANG
Publication Type:Journal Article
Keywords: Astragalus membranaceus; Codonopsis pilosula; ISSR; genetic relationship
From: Chinese Journal of Information on Traditional Chinese Medicine 2015;(2):79-82
CountryChina
Language:Chinese
Abstract: Objective To investigate the genetic relationships of Astragalus membranaceus and Codonopsis pilosula from the different cultivars (varieties). Methods Twenty-five samples of A. membranaceus and seventeen samples of C. Pilosula were amplified by ISSR-PCR. Genetic diversity and genetic distance were analyzed by Popgen32. UPGMA dendrogram relationship was clustered by NTSYS. Results Totally 62 and 100 loci were detected respectively from A. membranaceus and C. pilosula. All percentages of polymorphic loci (PPL) were 100%. Shannon’s information indexes (I) were 0.537 6 and 0.472 7. Nei’s genetic diversity indexes (H) were 0.361 3 and 0.307 4. A. membranaceus was divided into two groups in genetic distance 0.59, and C. pilosula was alike in 0.50. Conclusion Different cultivars (varieties) of A. membranaceus and C. pilosula had rich genetic diversities among species, and had large genetic differences.