Capsazepine in rats′lateral ventricles:Effect of different doses on LPS-induced fever

VernacularTitle:脑室给予不同剂量的Capsazepine对LPS致大鼠发热过程的影响
Author: Khanal BIBEK ; Bei SHI ; Paudel PUJA ; Pan ZHANG ; Xin QIN ; Yu CAO
Publication Type:Journal Article
Keywords: fever; transient receptor potential va-nilloid receptor 1; Capsazepine; LPS; hypothalamic; Ca2+
From: Chinese Pharmacological Bulletin 2015;(1):35-38
CountryChina
Language:Chinese
Abstract: Aim To observe the effects of the transient receptor potential vanilloid receptor 1 ( TRPV1 ) antago-nist Capsazepine on the LPS-induced fever process. Methods Three doses of Capsazepine were respec-tively injected into rats’ lateral ventricles before the in-traperitoneal injection of LPS caused fever. An emitter was implanted inside the rat’ s abdominal cavity to mo-nitor the body temperature changes during the fever process. Then the changes of calcium ion in the hypo-thalamus were detected by fluorescence and the expres-sions of TRPV1 protein were detected by Western blot. Results The higher dosage of Capsazepine was injec-ted, the higher the level of LPS-induced fever was, and the lower the concentration of calcium ion in the hypothalamus as well as the TRPV1 expressions were. Conclusion Capsazepine could increase the level of fever induced by LPS via acting on the rats’ hypothala-mus. This effect might be related to the functions of TRPV1 .