Effect of ginsenoside Rg1 on JNK and oligomeric Aβ1-42-induced apoptosis
10.3969/j.issn.1001-1978.2015.01.014
- VernacularTitle:人参皂苷Rg1对寡聚肽Aβ1-42增加JNK活性及诱导凋亡的影响
- Author:
Tianwen HUANG
;
Raoli HE
;
Meng ZHOU
;
Jing ZHANG
;
Xiaochun CHEN
- Publication Type:Journal Article
- Keywords:
ginsenoside Rg1;
cortical neuron;
JNK;
phosphorylation;
oligomeric Aβ1-42;
caspase 3;
apop-tosis
- From:
Chinese Pharmacological Bulletin
2015;(1):60-63
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the possible protective effect of ginsenoside Rg1 on oligomeric Aβ1-42 induced apoptosis and its possible mechanism. Methods The damage was induced by oligomeric Aβ1-42 in primary cortical neurons. Cells were incubated in the absence or presence of Aβ, or co-incubated in sp600125 with Aβ, or pre-incubated in ginsenoside Rg1 then co-incu-bated in Aβ. The p-JNK, JNK, caspase-3 activity and TUNEL-positive cells were detected. Results In Aβ1-42 treated group, the ratio of p-JNK/JNK level was increased more than that in non-treated group for 15 min. However, in neurons preincubated with (2. 5, 5, 10 μmol·L-1 ) ginsenoside Rg1 and then co-incuba-ted with 5 μmol·L-1 oligomeric Aβ1-42 , the p-JNK/JNK ratio, caspase-3 activity and TUNEL positive neu-rons were significantly decreased compared with those of Aβ1-42 treated group. Conclusion Ginsenoside Rg1 can attenuate the oligomeric Aβ1-42-induced apop-tosis by JNK pathway.
