Determination of plasma protein binding rate of five components in Eucommia ulmoides extract
10.3969/j.issn.1001-1978.2015.01.028
- VernacularTitle:杜仲提取物中五个成分血浆蛋白结合率的测定
- Author:
Xu CAO
;
Yumin XIE
;
Di ZHU
;
Pengcheng CHEN
;
Zipeng GONG
;
Aimin WANG
- Publication Type:Journal Article
- Keywords:
Eucommia ulmoides;
geniposidic acid;
protocatechuic acid;
chlorogenic acid;
pinoresinol diglucoside;
pinoresinol singleglucoside;
equilibrium dialysis method;
UPLC-MS/MS
- From:
Chinese Pharmacological Bulletin
2015;(1):131-135
- CountryChina
- Language:Chinese
-
Abstract:
Aim To determine the plasma protein binding rate of five components of Eucommia ulmoides extract. Methods The equilibrium dialysis method was used to study the plasma protein binding rate. The plasma samples were extracted by protein precipitation with methanol. With the use of puerarin as the internal standard, UPLC-MS/MS was carried out to determine the concentration of the five compounds in and out of the dialysis membrane. Results The average plasma protein binding rates of five compounds on the area of the concentration which was determinate were as fol-lows, respectively: geniposidic acid was ( 25. 77 ± 2. 68 )%, protocatechuic acid was ( 57. 54 ± 3. 79)%, chlorogenic acid was (53. 91 ± 3. 00)%, pinoresinol diglucoside was (24. 15 ± 4. 92)%, and pinoresinol singleglucoside was (49. 78 ± 3. 61)%. Conclusions The results show that the binding percentage of geniposidic acid and pinoresinol diglucoside is relatively low, but the binding rate of the others with rat plasma protein is moderate.
